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اسم البحث العلمي	EFFECT OF HIGH ALTITUDE HYPOXIA ON THE RAT OVARY MORPHOLOGY AND OOGENESIS
نوع البحث	دكتوراة
إسم الطالب	Ali Gadkarim Eldai Salih
المشرف	Prof. Qurashi M. Ali
تاريخ البحث	2018-01-01
الكلية	الطب
ملخص البحث بالعربى
ملخص البحث بالانجليزى	This study was designed to evaluate the effects of hypoxia on the ovarian structures, various stages of follicular maturation and probability of adaptation when the rats returned to their low altitude environment. Investigation of the ovarian structures was done using light and electron microscopy, histometeric analysis, hemoglobin and packed cell volume, hormonal assay in addition to measurement of ovarian oxidative stress. The study was carried out at Abha-Saudi Arabia representing the high altitude (HA), 2800 - 3150 meters above sea level; and Jazan, 43 meters above sea level, representing the low altitude (LA). Climatic conditions in these two cities are as follows: summer temperature varies between 23- 45°C in Jazan and 16-28°C in Abha. The winter temperature is 23-25°C in Jazan and 5-15°C in Abha. The relative humidity is 74% in Jazan and 25% in Abha. The atmospheric O2 tension is 159 mmHg in Jazan and 120 mmHg in Abha. The barometric pressure in Jazan is 759 mmHg and 590 mmHg in Abha. The study has been carried out during March to May 2015. 65 female rats were used in this study. 5 rats as control and kept at LA up to the end of experiments ; groups from1- 6 each of 5 rats and group 7 of 30 rats were kept at HA for periods of 5, 10, 15, 20, 25 and 30 days vi respectively to study the effect of hypoxia on various parameters mentioned above. 30 rats of group 7 were taken to LA, divided into 6 groups and kept for the same pervious period of hypoxic rats, 5, 10, 15, 20,25,30 days to see if the effects of hypoxia on the various parameters were reversed. Exposure of rats to HA resulted in atrophy and degeneration of the various ovarian follicles and interstitial tissue together with a decrease in cellularity in additions to presence of many vacuolation within and among the folliculi and many atretic follicles when exposed to HA environment. Quantitative assessment of primordial and antral follicles showed marked decrease when exposed to HA and improved in LA environment, whereas the assessment of primary and secondary follicles revealed increase when exposed to HA environment, and started to ameliorate gradually when returned to LA environment. Furthermore, formation of large blood vessels in the interstitial tissue, probably indicating vasodilatation, to compensate for the low partial pressure of oxygen at HA. Increase in the levels of the hemoglobin and PCV values as compensatory mechanisms for adaptation to HA environment were observed. Also there was a drop in the levels of FSH, LH and Estrogen hormones at hypoxic rats. Normal values were noticed at reversal groups of rats. Induction of oxidative vii stress was manifested by decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities. Exposure of rats to LA prevented ovarian oxidative stress. The present study showed that the adverse effect of hypobaric hypoxia in ovarian morphology, or oxidative stress and hypophysis–gonadal hormonal axis in rats is transient and reversible. The study recommends that further research should concentrate on low altitude as therapy and on the use of antioxidants for overcoming the oxidative stress.

المراكز

المعاهد
- معهد البحوث والدراسات الجنائية والاجتماعية
- علوم الادلة الجنائية

السودان - الخرطوم- بري - شارع النيل ص.ب: 55
تلفون:273598-273597 فاكس:263596
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